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N‐Heterocyclic Carbene–Gold(I) Complexes Conjugated to a Leukemia‐Specific DNA Aptamer for Targeted Drug Delivery
Author(s) -
Niu Weijia,
Chen Xigao,
Tan Weihong,
Veige Adam S.
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201602702
Subject(s) - bioconjugation , aptamer , conjugate , chemistry , conjugated system , flow cytometry , carbene , combinatorial chemistry , microbiology and biotechnology , biochemistry , biology , organic chemistry , mathematical analysis , catalysis , mathematics , polymer
This report describes the synthesis and characterization of novel N‐heterocyclic carbene (NHC)–gold(I) complexes and their bioconjugation to the CCRF‐CEM‐leukemia‐specific aptamer sgc8c. Successful bioconjugation was confirmed by the use of fluorescent tags on both the NHC–Au I complex and the aptamer. Cell‐viability assays indicated that the NHC–Au I –aptamer conjugate was more cytotoxic than the NHC–gold complex alone. A combination of flow cytometry, confocal microscopy, and cell‐viability assays provided clear evidence that the NHC–Au I –aptamer conjugate was selective for targeted CCRF‐CEM leukemia cells.