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Fast Quantitation of Target Analytes in Small Volumes of Complex Samples by Matrix‐Compatible Solid‐Phase Microextraction Devices
Author(s) -
PiriMoghadam Hamed,
Ahmadi Fardin,
GómezRíos German Augusto,
Boyacı Ezel,
ReyesGarcés Nathaly,
Aghakhani Ali,
Bojko Barbara,
Pawliszyn Janusz
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201601476
Subject(s) - solid phase microextraction , analyte , chromatography , matrix (chemical analysis) , materials science , complex matrix , sample preparation , extraction (chemistry) , mass spectrometry , polypyrrole , analytical chemistry (journal) , chemistry , gas chromatography–mass spectrometry , polymer , composite material , polymerization
Herein we report the development of solid‐phase microextraction (SPME) devices designed to perform fast extraction/enrichment of target analytes present in small volumes of complex matrices (i.e. V ≤10 μL). Micro‐sampling was performed with the use of etched metal tips coated with a thin layer of biocompatible nano‐structured polypyrrole (PPy), or by using coated blade spray (CBS) devices. These devices can be coupled either to liquid chromatography (LC), or directly to mass spectrometry (MS) via dedicated interfaces. The reported results demonstrated that the whole analytical procedure can be carried out within a few minutes with high sensitivity and quantitation precision, and can be used to sample from various biological matrices such as blood, urine, or Allium cepa L single‐cells.