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High‐Throughput Measurement of Binding Kinetics by mRNA Display and Next‐Generation Sequencing
Author(s) -
JalaliYazdi Farzad,
Huong Lai Lan,
Takahashi Terry T.,
Roberts Richard W.
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201600077
Subject(s) - throughput , kinetics , computational biology , messenger rna , dna sequencing , high throughput screening , chemistry , biology , computer science , biochemistry , dna , gene , physics , telecommunications , quantum mechanics , wireless
There is great demand for high‐throughput methods to characterize ligand affinity. By combining mRNA display with next‐generation sequencing, we determined the kinetic on‐ and off‐rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement.
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