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Modified RNAs in CRISPR/Cas9: An Old Trick Works Again
Author(s) -
Latorre Alfonso,
Latorre Ana,
Somoza Álvaro
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201512002
Subject(s) - crispr , cas9 , endonuclease , rna , guide rna , computational biology , genome editing , crispr interference , rna interference , trans activating crrna , gene , biology , chemistry , computer science , genetics
Old tricks, new dog : CRISPR/Cas9 is a powerful tool for gene editing that requires an endonuclease (Cas9) and RNA strands. It has been shown that chemical modification of the RNA structures, an approach that has been used to improve the efficiency of RNA interference, can also be applied to enhance the activity of CRISPR/Cas9 and reduce its off‐target effects.