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Molecular Imaging of Biological Samples on Nanophotonic Laser Desorption Ionization Platforms
Author(s) -
Stopka Sylwia A.,
Rong Charles,
Korte Andrew R.,
Yadavilli Sridevi,
Nazarian Javad,
Razunguzwa Trust T.,
Morris Nicholas J.,
Vertes Akos
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201511691
Subject(s) - ionization , mass spectrometry imaging , matrix assisted laser desorption electrospray ionization , atmospheric pressure laser ionization , laser , biomolecule , mass spectrometry , matrix assisted laser desorption/ionization , nanophotonics , chemistry , materials science , desorption , analytical chemistry (journal) , ion , photoionization , optoelectronics , optics , nanotechnology , physics , thermal ionization mass spectrometry , chromatography , organic chemistry , adsorption
Mass spectrometry imaging (MSI) is a comprehensive tool for the analysis of a wide range of biomolecules. The mainstream method for molecular MSI is matrix‐assisted laser desorption ionization, however, the presence of a matrix results in spectral interferences and the suppression of some analyte ions. Herein we demonstrate a new matrix‐free MSI technique using nanophotonic ionization based on laser desorption ionization (LDI) from a highly uniform silicon nanopost array (NAPA). In mouse brain and kidney tissue sections, the distributions of over 80 putatively annotated molecular species are determined with 40 μm spatial resolution. Furthermore, NAPA‐LDI‐MS is used to selectively analyze metabolites and lipids from sparsely distributed algal cells and the lamellipodia of human hepatocytes. Our results open the door for matrix‐free MSI of tissue sections and small cell populations by nanophotonic ionization.