Premium
A Universal Polymerase Chain Reaction Developer
Author(s) -
Valentini Paola,
Pompa Pier Paolo
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201511010
Subject(s) - polymerase chain reaction , dna , naked eye , colloidal gold , microbiology and biotechnology , genomic dna , computational biology , gel electrophoresis , chemistry , nanotechnology , biology , chromatography , gene , nanoparticle , materials science , detection limit , biochemistry
The versatility of PCR, the gold standard for amplification of DNA targets, is hampered by the laborious, multi‐step detection based on gel electrophoresis. We propose a one‐step, one‐tube method for the rapid (5 min) naked‐eye detection of PCR products, based on controlled aggregation of gold nanoparticles. Our method is universal, instrument‐free, and ultra‐sensitive, as it could detect as low as 0.01 zeptomoles of HIV template DNA in an excess of interfering human genomic DNA.