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Solid‐State NMR of PEGylated Proteins
Author(s) -
Ravera Enrico,
Ciambellotti Silvia,
Cerofolini Linda,
Martelli Tommaso,
Kozyreva Tatiana,
Bernacchioni Caterina,
Giuntini Stefano,
Fragai Marco,
Turano Paola,
Luchinat Claudio
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201510148
Subject(s) - biomedicine , solid state nuclear magnetic resonance , characterization (materials science) , computational biology , chemistry , materials science , nanotechnology , nuclear magnetic resonance , bioinformatics , biology , physics
PEGylated proteins are widely used in biomedicine but, in spite of their importance, no atomic‐level information is available since they are generally resistant to structural characterization approaches. PEGylated proteins are shown here to yield highly resolved solid‐state NMR spectra, which allows assessment of the structural integrity of proteins when PEGylated for therapeutic or diagnostic use.