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Bioconjugating Thiols to Poly(acrylamide) Gels for Cell Culture Using Methylsulfonyl Co‐monomers
Author(s) -
Farrukh Aleeza,
Paez Julieta I.,
Salierno Marcelo,
del Campo Aránzazu
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201509986
Subject(s) - acrylamide , bioconjugation , self healing hydrogels , covalent bond , monomer , chemistry , swelling , polymer chemistry , chemical engineering , adsorption , materials science , polymer , combinatorial chemistry , organic chemistry , engineering
Poly(acrylamide) P(AAm) gels have become relevant model substrates to study cell response to the mechanical and biochemical properties of the cellular microenvironment. However, current bioconjugation strategies to functionalize P(AAm) gels, mainly using photoinduced arylazide coupling, show poor specificity and hinder conclusive studies of material properties and cellular responses. We describe methylsulfonyl‐containing P(AAm) hydrogels for cell culture. These gels allow easy, specific and functional covalent coupling of thiol containing bioligands in tunable concentrations under physiological conditions, while retaining the same swelling, porosity, cytocompatibility, and low protein adsorption of P(AAm) gels. These materials allow quantitative and standardized studies of cell‐materials interactions with P(AAm) gels.