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Multiscale Origami Structures as Interface for Cells
Author(s) -
Angelin Alessandro,
Weigel Simone,
Garrecht Ruben,
Meyer Rebecca,
Bauer Jens,
Kumar Ravi Kapoor,
Hirtz Michael,
Niemeyer Christof M.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201509772
Subject(s) - dna origami , nanotechnology , micropatterning , interface (matter) , dna , nanostructure , nanoscopic scale , materials science , chemistry , wetting , biochemistry , sessile drop technique , composite material
A DNA‐based platform was developed to address fundamental aspects of early stages of cell signaling in living cells. By site‐directed sorting of differently encoded, protein‐decorated DNA origami structures on DNA microarrays, we combine the advantages of the bottom‐up self‐assembly of protein–DNA nanostructures and top‐down micropatterning of solid surfaces to create multiscale origami structures as interface for cells (MOSAIC). In a proof‐of‐principle, we use this technology to analyze the activation of epidermal growth factor (EGF) receptors in living MCF7 cells using DNA origami structures decorated on their surface with distinctive nanoscale arrangements of EGF ligand entities. MOSAIC holds the potential to present to adhered cells well‐defined arrangements of ligands with full control over their number, stoichiometry, and precise nanoscale orientation. It therefore promises novel applications in the life sciences, which cannot be tackled by conventional technologies.