Premium
Genetic Optimization of Metalloenzymes: Enhancing Enzymes for Non‐Natural Reactions
Author(s) -
Hyster Todd K.,
Ward Thomas R.
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201508816
Subject(s) - reactivity (psychology) , coordination sphere , chemistry , artificial enzyme , combinatorial chemistry , biochemical engineering , nanotechnology , catalysis , computer science , materials science , metal , organic chemistry , engineering , medicine , alternative medicine , pathology
Artificial metalloenzymes have received increasing attention over the last decade as a possible solution to unaddressed challenges in synthetic organic chemistry. Whereas traditional transition‐metal catalysts typically only take advantage of the first coordination sphere to control reactivity and selectivity, artificial metalloenzymes can modulate both the first and second coordination spheres. This difference can manifest itself in reactivity profiles that can be truly unique to artificial metalloenzymes. This Review summarizes attempts to modulate the second coordination sphere of artificial metalloenzymes by using genetic modifications of the protein sequence. In doing so, successful attempts and creative solutions to address the challenges encountered are highlighted.