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A High‐Throughput Platform for Formulating and Screening Multifunctional Nanoparticles Capable of Simultaneous Delivery of Genes and Transcription Factors
Author(s) -
Liu Yang,
Du Juanjuan,
Choi Jinsil,
Chen KuanJu,
Hou Shuang,
Yan Ming,
Lin WeiYu,
Chen Kevin Sean,
Ro Tracy,
Lipshutz Gerald S.,
Wu Lily,
Shi Linqi,
Lu Yunfeng,
Tseng HsianRong,
Wang Hao
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201507546
Subject(s) - reprogramming , gene delivery , computational biology , synthetic biology , transcription factor , nanotechnology , high throughput screening , in vivo , gene , biology , bioinformatics , materials science , genetic enhancement , microbiology and biotechnology , biochemistry
Simultaneous delivery of multiple genes and proteins (e.g., transcription factors; TFs) is an emerging issue surrounding therapeutic research due to their ability to regulate cellular circuitry. Current gene and protein delivery strategies, however, are based on slow batch synthesis, which is ineffective, poorly controlled, and incapable of simultaneous delivery of both genes and proteins with synergistic functions. Consequently, advances in this field have been limited to in vitro studies. Here, by integrating microfluidic technologies with a supramolecular synthetic strategy, we present a high‐throughput approach for formulating and screening multifunctional supramolecular nanoparticles (MFSNPs) self‐assembled from a collection of functional modules to achieve simultaneous delivery of one gene and TF with unprecedented efficiency both in vitro and in vivo. We envision that this new approach could open a new avenue for immunotherapy, stem cell reprogramming, and other therapeutic applications.