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Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP‐Supported Solid‐State NMR Spectroscopy
Author(s) -
Jantschke Anne,
Koers Eline,
Mance Deni,
Weingarth Markus,
Brunner Eike,
Baldus Marc
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201507327
Subject(s) - supramolecular chemistry , solid state nuclear magnetic resonance , spectroscopy , solid state , nuclear magnetic resonance spectroscopy , diatom , materials science , chemistry , chemical engineering , nanotechnology , crystallography , nuclear magnetic resonance , organic chemistry , geology , molecule , engineering , oceanography , physics , quantum mechanics
Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP‐supported ssNMR approach assisted by microscopy, MS, and MD simulations was applied to study the structural organization of intact biosilica. For the first time, the secondary structure elements of tightly biosilica‐associated native proteins in diatom biosilica were characterized in situ. Our data suggest that these proteins are rich in a limited set of amino acids and adopt a mixture of random‐coil and β‐strand conformations. Furthermore, biosilica‐associated long‐chain polyamines and carbohydrates were characterized, thereby leading to a model for the supramolecular organization of intact biosilica.