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The cis ‐Diammineplatinum(II) Complex of Curcumin: A Dual Action DNA Crosslinking and Photochemotherapeutic Agent
Author(s) -
Mitra Koushambi,
Gautam Srishti,
Kondaiah Paturu,
Chakravarty Akhil R.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201507281
Subject(s) - curcumin , platinum , chemistry , adduct , comet assay , dna , dna damage , fluorescence , stereochemistry , photochemistry , biochemistry , organic chemistry , physics , quantum mechanics , catalysis
[Pt(cur)(NH 3 ) 2 ](NO 3 ) ( 1 ), a curcumin‐bound cis‐diammineplatinum(II) complex, nicknamed Platicur, as a novel photoactivated chemotherapeutic agent releases photoactive curcumin and an active platinum(II) species upon irradiation with visible light. The hydrolytic instability of free curcumin reduces upon binding to platinum(II). Interactions of 1 with 5′‐GMP and ct‐DNA indicated formation of platinum‐bound DNA adducts upon exposure to visible light ( λ =400–700 nm). It showed apoptotic photocytotoxicity in cancer cells (IC 50 ≈15 μ M ), thus forming ⋅ OH, while remaining passive in the darkness (IC 50 >200 μ M ). A comet assay and platinum estimation suggest Pt–DNA crosslink formation. The fluorescence microscopic images showed cytosolic localization of curcumin, thus implying possibility of dual action as a chemo‐ and phototherapeutic agent.