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Cover Picture: A Rapid, Amplification‐Free, and Sensitive Diagnostic Assay for Single‐Step Multiplexed Fluorescence Detection of MicroRNA (Angew. Chem. Int. Ed. 34/2015)
Author(s) -
Jin Zongwen,
Geißler Daniel,
Qiu Xue,
Wegner K. David,
Hildebrandt Niko
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201506281
Subject(s) - förster resonance energy transfer , fluorescence , chemistry , microrna , multiplexing , luminescent measurements , luminescence , biosensor , nanotechnology , biophysics , combinatorial chemistry , computational biology , materials science , biochemistry , computer science , biology , physics , optoelectronics , gene , telecommunications , quantum mechanics
The simple, sensitive, and selective detection of short‐sequence microRNAs is very challenging. In their Communication on page 10024 ff. N. Hildebrandt and co‐workers present a single‐step “mix‐and‐measure” FRET assay for the multiplexed detection of different microRNAs. The careful design of the biophotonic probe and time‐gated FRET from a luminescent Tb complex to three difference fluorescent dyes are crucial to this microRNA test for multiplexed clinical diagnostics.