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A Set of Homo‐Oligomeric Standards Allows Accurate Protein Counting
Author(s) -
Finan Kieran,
Raulf Anika,
Heilemann Mike
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201505664
Subject(s) - microscopy , selection (genetic algorithm) , stoichiometry , chemistry , fluorescence microscope , fluorescence , computational biology , computer science , biological system , nanotechnology , biology , materials science , physics , artificial intelligence , optics , organic chemistry
Techniques based on fluorescence microscopy are increasingly used to count proteins in cells, but few stoichiometrically well‐defined standards are available to test their accuracy. A selection of bacterial homo‐oligomers were developed that contain 10–24 subunits and fully assemble when expressed in mammalian cells, and they can be used to easily validate/calibrate molecular counting methods. The utility of these standards was demonstrated by showing that nuclear pores contain 32 copies of the Nup107 complex.