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A Versatile Approach Towards Nucleobase‐Modified Aptamers
Author(s) -
Tolle Fabian,
Brändle Gerhard M.,
Matzner Daniel,
Mayer Günter
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201503652
Subject(s) - aptamer , nucleobase , nucleic acid , chemical space , modular design , computer science , combinatorial chemistry , nanotechnology , computational biology , chemistry , dna , drug discovery , biology , materials science , genetics , biochemistry , operating system
A novel and versatile method has been developed for modular expansion of the chemical space of nucleic acid libraries, thus enabling the generation of nucleobase‐modified aptamers with unprecedented recognition properties. Reintroduction of the modification after enzymatic replication gives broad access to many chemical modifications. This wide applicability, which is not limited to a single modification, will rapidly advance the application of in vitro selection approaches beyond what is currently feasible and enable the generation of aptamers to many targets that have so far not been addressable.