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A Photoactivatable AIE Polymer for Light‐Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking
Author(s) -
Yuan Youyong,
Zhang ChongJing,
Liu Bin
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201503640
Subject(s) - gene delivery , chemistry , polymer , unpacking , dna , nucleic acid , conjugated system , biophysics , linker , fluorescence , gene , rolling circle replication , polylysine , genetic enhancement , photochemistry , biochemistry , biology , organic chemistry , physics , linguistics , computer science , operating system , dna replication , philosophy , quantum mechanics
Endo/lysosomal escape of gene vectors and the subsequent unpacking of nucleic acids in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation‐induced emission (AIE) characteristics and oligoethylenimine (OEI) conjugated via an aminoacrylate (AA) linker that can be cleaved by reactive oxygen species (ROS). In aqueous media, the polymer could self‐assemble into bright red fluorescent nanoparticles (NPs), which can efficiently bind to DNA through electrostatic interaction for gene delivery. Upon visible light irradiation, the generated ROS can break the endo/lysosomal membrane and the polymer, resulting in light‐controlled endo/lysosomal escape and unpacking of DNA for efficient gene delivery. The smart polymer represents the first successful gene vector to simultaneously address both challenges with a single light excitation process.