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SLAP: Small Labeling Pair for Single‐Molecule Super‐Resolution Imaging
Author(s) -
Wieneke Ralph,
Raulf Anika,
Kollmannsperger Alina,
Heilemann Mike,
Tampé Robert
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201503215
Subject(s) - resolution (logic) , superresolution , materials science , nanotechnology , computer science , computer vision , artificial intelligence , image (mathematics)
Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. A sensitive and efficient modular labeling approach (SLAP) was developed on the basis of a synthetic small‐molecule recognition unit (Ni‐ tris NTA) and the genetically encoded minimal protein His 6‐10 ‐tag. High‐density protein tracing by SLAP was demonstrated. This technique allows super‐resolution fluorescence imaging and fulfills the necessary sampling criteria for single‐molecule localization‐based imaging techniques. It avoids masking by large probes, for example, antibodies, and supplies sensitive, precise, and robust size analysis of protein clusters (nanodomains).