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Minimal Synthetic Cells to Study Integrin‐Mediated Adhesion
Author(s) -
Frohnmayer Johannes P.,
Brüggemann Dorothea,
Eberhard Christian,
Neubauer Stefanie,
Mollenhauer Christine,
Boehm Heike,
Kessler Horst,
Geiger Benjamin,
Spatz Joachim P.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201503184
Subject(s) - integrin , fibronectin , cell adhesion , adhesion , microbiology and biotechnology , extracellular matrix , chemistry , biophysics , transmembrane protein , liposome , cell , biochemistry , biology , receptor , organic chemistry
To shed light on cell‐adhesion‐related molecular pathways, synthetic cells offer the unique advantage of a well‐controlled model system with reduced molecular complexity. Herein, we show that liposomes with the reconstituted platelet integrin α IIb β 3 as the adhesion‐mediating transmembrane protein are a functional minimal cell model for studying cellular adhesion mechanisms in a defined environment. The interaction of these synthetic cells with various extracellular matrix proteins was analyzed using a quartz crystal microbalance with dissipation monitoring. The data indicated that integrin was functionally incorporated into the lipid vesicles, thus enabling integrin‐specific adhesion of the engineered liposomes to fibrinogen‐ and fibronectin‐functionalized surfaces. Then, we were able to initiate the detachment of integrin liposomes from these surfaces in the presence of the peptide GRGDSP, a process that is even faster with our newly synthesized peptide mimetic SN529, which specifically inhibits the integrin α IIb β 3 .