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Oxidative Decarboxylation of Short‐Chain Fatty Acids to 1‐Alkenes
Author(s) -
Dennig Alexander,
Kuhn Miriam,
Tassoti Sebastian,
Thiessenhusen Anja,
Gilch Stefan,
Bülter Thomas,
Haas Thomas,
Hall Mélanie,
Faber Kurt
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201502925
Subject(s) - decarboxylation , chemistry , oxidative decarboxylation , nad+ kinase , oxidative phosphorylation , formate , electron transfer , enzyme , organic chemistry , catalysis , biochemistry
The enzymatic oxidative decarboxylation of linear short‐chain fatty acids (C4:0–C9:0) employing the P450 monooxygenase OleT, O 2 as the oxidant, and NAD(P)H as the electron donor gave the corresponding terminal C 3 to C 8 alkenes with product titers of up to 0.93 g L −1 and TTNs of >2000. Key to this process was the construction of an efficient electron‐transfer chain employing putidaredoxin CamAB in combination with NAD(P)H recycling at the expense of glucose, formate, or phosphite. This system allows for the biocatalytic production of industrially important 1‐alkenes, such as propene and 1‐octene, from renewable resources for the first time.