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Reversal of a Single Base‐Pair Step Controls Guanine Photo‐Oxidation by an Intercalating Ruthenium(II) Dipyridophenazine Complex
Author(s) -
Keane Páraic M.,
Poynton Fergus E.,
Hall James P.,
Sazanovich Igor V.,
Towrie Michael,
Gunnlaugsson Thorfinnur,
Quinn Susan J.,
Cardin Christine J.,
Kelly John M.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201502608
Subject(s) - guanine , intercalation (chemistry) , oligonucleotide , chemistry , ruthenium , stereochemistry , dna , crystallography , nanosecond , photochemistry , biochemistry , nucleotide , inorganic chemistry , optics , laser , physics , gene , catalysis
Small changes in DNA sequence can often have major biological effects. Here the rates and yields of guanine photo‐oxidation by Λ‐[Ru(TAP) 2 (dppz)] 2+ have been compared in 5′‐{CCGG AT CCGG} 2 and 5′‐{CCGG TA CCGG} 2 using pico/nanosecond transient visible and time‐resolved IR (TRIR) spectroscopy. The inefficiency of electron transfer in the TA sequence is consistent with the 5′‐TA‐3′ versus 5′‐AT‐3′ binding preference predicted by X‐ray crystallography. The TRIR spectra also reveal the differences in binding sites in the two oligonucleotides.