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Structural Transformation: Assembly of an Otherwise Inaccessible DNA Nanocage
Author(s) -
Li Yulin,
Tian Cheng,
Liu Zhiyu,
Jiang Wen,
Mao Chengde
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201500755
Subject(s) - nanocages , transformation (genetics) , dna , dna origami , dna nanotechnology , nanotechnology , materials science , chemistry , biochemistry , gene , catalysis
A strategy of structural transformation for the assembly of DNA nanocages that can not be assembled directly is described. In this strategy, a precursor DNA nanocage is assembled first and then is isothermally transformed into a desired, complicated nanocage. A dramatic, conformational change accompanies the transformation. This strategy has been proven to be successful by native polyacrylamide gel electrophoresis (PAGE) and cryogenic electron microscopy (cryoEM) imaging. We expect that the strategy of structural transformation will be useful for the assembly of many otherwise inaccessible DNA nanostructures and help to increase the structural complexity of DNA nanocages.