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One‐Step Formation of “Chain‐Armor”‐Stabilized DNA Nanostructures
Author(s) -
Cassinelli Valentina,
Oberleitner Birgit,
Sobotta Jessica,
Nickels Philipp,
Grossi Guido,
Kempter Susanne,
Frischmuth Thomas,
Liedl Tim,
Manetto Antonio
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201500561
Subject(s) - oligonucleotide , dna , catenane , template , dna origami , nanostructure , click chemistry , covalent bond , nanotechnology , chemistry , nanoscopic scale , dna nanotechnology , helix (gastropod) , drug delivery , linker , materials science , crystallography , combinatorial chemistry , molecule , biochemistry , organic chemistry , biology , ecology , snail , computer science , operating system
DNA‐based self‐assembled nanostructures are widely used to position organic and inorganic objects with nanoscale precision. A particular promising application of DNA structures is their usage as programmable carrier systems for targeted drug delivery. To provide DNA‐based templates that are robust against degradation at elevated temperatures, low ion concentrations, adverse pH conditions, and DNases, we built 6‐helix DNA tile tubes consisting of 24 oligonucleotides carrying alkyne groups on their 3′‐ends and azides on their 5′‐ends. By a mild click reaction, the two ends of selected oligonucleotides were covalently connected to form rings and interlocked DNA single strands, so‐called DNA catenanes. Strikingly, the structures stayed topologically intact in pure water and even after precipitation from EtOH. The structures even withstood a temperature of 95 °C when all of the 24 strands were chemically interlocked.