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Development of Imidazoline‐2‐Thiones Based Two‐Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co‐Culture System
Author(s) -
Xu Qingling,
Heo Cheol Ho,
Kim Gyoungmi,
Lee Hyo Won,
Kim Hwan Myung,
Yoon Juyoung
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201500537
Subject(s) - hypochlorite , fluorescence , chemistry , two photon excitation microscopy , imidazoline receptor , ion , photochemistry , inorganic chemistry , biology , organic chemistry , optics , physics , endocrinology
We designed and prepared the imidazoline‐2‐thione containing OCl − probes, PIS and NIS , which operate through specific reactions with OCl − that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl − generation in macrophages in a co‐culture system. We have also employed two‐photon microscopy and PIS to image OCl − in live cells and tissues, indicating that this probe could have wide biological applications.