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Simultaneous Capture, Detection, and Inactivation of Bacteria as Enabled by a Surface‐Enhanced Raman Scattering Multifunctional Chip
Author(s) -
Wang Houyu,
Zhou Yanfeng,
Jiang Xiangxu,
Sun Bin,
Zhu Ying,
Wang Hui,
Su Yuanyuan,
He Yao
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201412294
Subject(s) - raman scattering , detection limit , wafer , etching (microfabrication) , chemistry , biosensor , reproducibility , nanotechnology , silver nanoparticle , chip , relative standard deviation , raman spectroscopy , materials science , nanoparticle , nuclear chemistry , chromatography , optics , physics , electrical engineering , engineering , layer (electronics)
Abstract Herein, we present a multifunctional chip based on surface‐enhanced Raman scattering (SERS) that effectively captures, discriminates, and inactivates pathogenic bacteria. The developed SERS chip is made of a silicon wafer decorated with silver nanoparticles and modified with 4‐mercaptophenylboronic acid (4‐MPBA). It was prepared in a straightforward manner by chemical reduction assisted by hydrogen fluoride etching, followed by the conjugation of 4‐MPBA through AgS bonds. The dominant merits of the fabricated SERS chip include excellent reproducibility with a relative standard deviation (RSD) value smaller than 11.0 %, adaptable bacterial‐capture efficiency (ca. 60 %) at low concentrations (500–2000 CFU mL −1 ), a low detection limit (down to a concentration of 1.0×10 2 cells mL −1 ), and high antibacterial activity (an antibacterial rate of ca. 97 %). The SERS chip enabled sensitive and specific discrimination of Escherichia coli and Staphylococcus aureus from human blood.