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Live‐cell MRI with Xenon Hyper‐CEST Biosensors Targeted to Metabolically Labeled Cell‐Surface Glycans
Author(s) -
Witte Christopher,
Martos Vera,
Rose Honor May,
Reinke Stefan,
Klippel Stefan,
Schröder Leif,
Hackenberger Christian P. R.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201410573
Subject(s) - glycan , bioorthogonal chemistry , biosensor , xenon , cell , chemistry , sialic acid , biophysics , biochemistry , biology , click chemistry , combinatorial chemistry , glycoprotein , organic chemistry
The targeting of metabolically labeled glycans with conventional MRI contrast agents has proved elusive. In this work, which further expands the utility of xenon Hyper‐CEST biosensors in cell experiments, we present the first successful molecular imaging of such glycans using MRI. Xenon Hyper‐CEST biosensors are a novel class of MRI contrast agents with very high sensitivity. We designed a multimodal biosensor for both fluorescent and xenon MRI detection that is targeted to metabolically labeled sialic acid through bioorthogonal chemistry. Through the use of a state of the art live‐cell bioreactor, it was demonstrated that xenon MRI biosensors can be used to image cell‐surface glycans at nanomolar concentrations.