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EPR‐Based Approach for the Localization of Paramagnetic Metal Ions in Biomolecules
Author(s) -
Abdullin Dinar,
Florin Nicole,
Hagelueken Gregor,
Schiemann Olav
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201410396
Subject(s) - electron paramagnetic resonance , biomolecule , ion , metal ions in aqueous solution , pulsed epr , paramagnetism , azurin , trilateration , metal , chemistry , materials science , nanotechnology , nuclear magnetic resonance , copper , chemical physics , magnetic resonance imaging , physics , condensed matter physics , spin echo , medicine , organic chemistry , quantum mechanics , node (physics) , radiology
Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three‐dimensional fold of such biomolecules is therefore an important goal in understanding structure–function relationships. A trilateration approach for the localization of metal ions by means of long‐range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu 2+ center of azurin, and factors affecting the precision of the method are discussed.