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Fluorescence Microscopy Visualization of Contacts Between Objects
Author(s) -
Suhina Tomislav,
Weber Bart,
Carpentier Chantal E.,
Lorincz Kinga,
Schall Peter,
Bonn Daniel,
Brouwer Albert M.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201410240
Subject(s) - fluorescence , fluorescence microscope , molecule , microscopy , materials science , contact region , hertz , optics , surface (topology) , visualization , deformation (meteorology) , molecular physics , nanotechnology , physics , geometry , computer science , composite material , mathematics , layer (electronics) , artificial intelligence , quantum mechanics
The area of contact between two objects was detected by using the strong enhancement of the fluorescence of rigidochromic probe molecules attached to one of the surfaces. Confinement of the molecules suppresses nonradiative decay and turns on the fluorescence. The approach is demonstrated by imaging of the contact area of a plastic sphere in contact with a flat glass surface. Our results agree excellently with the prediction of Hertz’s classical theory based on elastic deformation.