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Quantitative Trace Analysis of Complex Mixtures Using SABRE Hyperpolarization
Author(s) -
Eshuis Nan,
van Weerdenburg Bram J. A.,
Feiters Martin C.,
Rutjes Floris P. J. T.,
Wijmenga Sybren S.,
Tessari Marco
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201409795
Subject(s) - hyperpolarization (physics) , spins , chemistry , molecule , analyte , analytical chemistry (journal) , spectral line , nuclear magnetic resonance spectroscopy , chemical physics , nuclear magnetic resonance , physics , chromatography , stereochemistry , condensed matter physics , organic chemistry , astronomy
Abstract Signal amplification by reversible exchange (SABRE) is an emerging nuclear spin hyperpolarization technique that strongly enhances NMR signals of small molecules in solution. However, such signal enhancements have never been exploited for concentration determination, as the efficiency of SABRE can strongly vary between different substrates or even between nuclear spins in the same molecule. The first application of SABRE for the quantitative analysis of a complex mixture is now reported. Despite the inherent complexity of the system under investigation, which involves thousands of competing binding equilibria, analytes at concentrations in the low micromolar range could be quantified from single‐scan SABRE spectra using a standard‐addition approach.