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Stiffness‐Dependent In Vitro Uptake and Lysosomal Acidification of Colloidal Particles
Author(s) -
Hartmann Raimo,
Weidenbach Marcel,
Neubauer Martin,
Fery Andreas,
Parak Wolfgang J.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201409693
Subject(s) - endocytosis , stiffness , colloid , particle (ecology) , biophysics , colloidal particle , particle size , chemistry , in vitro , constant (computer programming) , materials science , nanotechnology , composite material , biochemistry , biology , cell , ecology , computer science , programming language
The physico‐chemical properties of colloidal particles determine their uptake into cells. For a series of microparticles only one parameter, the mechanical stiffness, was varied, whereas other parameters such as size, shape, and charge were kept constant. The uptake was monitored in situ by analyzing individual particle trajectories including the progress of endocytosis, derived from local pH measurements around each particle. Evidence is presented that soft particles with low stiffness are transported faster to lysosomes than stiffer ones.