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Chip‐Based Free‐Flow Electrophoresis with Integrated Nanospray Mass‐Spectrometry
Author(s) -
Benz Christian,
Boomhoff Michael,
Appun Johannes,
Schneider Christoph,
Belder Detlev
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201409663
Subject(s) - free flow electrophoresis , mass spectrometry , capillary electrophoresis , chemistry , chromatography , electrophoresis , analytical chemistry (journal) , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , enzyme , biochemistry
Free‐flow electrophoresis is an ideal tool for preparative separations in continuous microflow. With the approach presented herein for coupling free‐flow electrophoresis and mass spectrometry it is now also possible to trace non‐fluorescent compounds and identify them by means of mass spectrometry. The functionality of the method and its potential as an integrated separation unit for microflow synthesis is demonstrated by application to a multicomponent [3+2]‐cycloannulation.