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Ion‐Selective Formation of a Guanine Quadruplex on DNA Origami Structures
Author(s) -
Olejko Lydia,
Cywinski Piotr J.,
Bald Ilko
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201409278
Subject(s) - dna origami , g quadruplex , guanine , dna , chemistry , template , steric effects , folding (dsp implementation) , molecule , ion , förster resonance energy transfer , nucleobase , biophysics , selectivity , nanotechnology , combinatorial chemistry , crystallography , stereochemistry , fluorescence , biochemistry , materials science , nucleotide , gene , biology , organic chemistry , physics , engineering , quantum mechanics , electrical engineering , catalysis
DNA origami nanostructures are a versatile tool that can be used to arrange functionalities with high local control to study molecular processes at a single‐molecule level. Here, we demonstrate that DNA origami substrates can be used to suppress the formation of specific guanine (G) quadruplex structures from telomeric DNA. The folding of telomeres into G‐quadruplex structures in the presence of monovalent cations (e.g. Na + and K + ) is currently used for the detection of K + ions, however, with insufficient selectivity towards Na + . By means of FRET between two suitable dyes attached to the 3′‐ and 5′‐ends of telomeric DNA we demonstrate that the formation of G‐quadruplexes on DNA origami templates in the presence of sodium ions is suppressed due to steric hindrance. Hence, telomeric DNA attached to DNA origami structures represents a highly sensitive and selective detection tool for potassium ions even in the presence of high concentrations of sodium ions.