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Long‐Lived Engineering of Glycans to Direct Stem Cell Fate
Author(s) -
Pulsipher Abigail,
Griffin Matthew E.,
Stone Shan E.,
HsiehWilson Linda C.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201409258
Subject(s) - glycan , microbiology and biotechnology , stem cell , embryonic stem cell , cell fate determination , induced pluripotent stem cell , cell , cellular differentiation , biology , chemistry , glycosaminoglycan , biochemistry , glycoprotein , transcription factor , gene
Glycans mediate many critical, long‐term biological processes, such as stem cell differentiation. However, few methods are available for the sustained remodeling of cells with specific glycan structures. A new strategy that enables the long‐lived presentation of defined glycosaminoglycans on cell surfaces using HaloTag proteins (HTPs) as anchors is reported. By controlling the sulfation patterns of heparan sulfate (HS) on pluripotent embryonic stem cell (ESC) membranes, it is demonstrated that specific glycans cause ESCs to undergo accelerated exit from self‐renewal and differentiation into neuronal cell types. Thus, the stable display of glycans on HTP scaffolds provides a powerful, versatile means to direct key signaling events and biological outcomes such as stem cell fate.