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Light‐Induced Protein Dimerization by One‐ and Two‐Photon Activation of Gibberellic Acid Derivatives in Living Cells
Author(s) -
Schelkle Korwin M.,
Griesbaum Tristan,
Ollech Dirk,
Becht Steffy,
Buckup Tiago,
Hamburger Manuel,
Wombacher Richard
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201409196
Subject(s) - gibberellic acid , chromosomal translocation , inducer , chemistry , photon , blue light , biophysics , photochemistry , biochemistry , biology , physics , optoelectronics , botany , optics , gene , germination
We developed a highly efficient system for light‐induced protein dimerization in live cells using photo‐caged derivatives of the phytohormone gibberellic acid (GA 3 ). We demonstrate the application of the photo‐activatable chemical inducer of dimerization (CID) for the control of protein translocation with high spatiotemporal precision using light as an external trigger. Furthermore, we present a new two‐photon (2P)‐sensitive caging group, whose exceptionally high two‐photon cross section allows the use of infrared light to efficiently unleash the active GA 3 for inducing protein dimerization in living cells.