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Direct Cytosolic Delivery of siRNA Using Nanoparticle‐Stabilized Nanocapsules
Author(s) -
Jiang Ying,
Tang Rui,
Duncan Bradley,
Jiang Ziwen,
Yan Bo,
Mout Rubul,
Rotello Vincent M.
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201409161
Subject(s) - nanocapsules , cytosol , chemistry , cytotoxicity , biophysics , gene knockdown , gene silencing , small interfering rna , nanocarriers , nanoparticle , cationic polymerization , colloidal gold , hek 293 cells , transfection , nanotechnology , microbiology and biotechnology , biochemistry , in vitro , apoptosis , materials science , enzyme , biology , gene , organic chemistry
The use of nanoparticle‐stabilized nanocapsules (NPSCs) for the direct cytosolic delivery of siRNA is reported. In this approach, siRNA is complexed with cationic arginine‐functionalized gold nanoparticles by electrostatic interactions, with the resulting ensemble self‐assembled onto the surface of fatty acid nanodroplets to form a NPSC/siRNA nanocomplex. The complex rapidly delivers siRNA into the cytosol through membrane fusion, a mechanism supported by cellular uptake studies. Using destabilized green fluorescent protein (deGFP) as a target, 90 % knockdown was observed in HEK293 cells. Moreover, the delivery of siRNA targeting polo‐like kinase 1 (siPLK1) efficiently silenced PLK1 expression in cancer cells with concomitant cytotoxicity.