Premium
Multiple Paramagnetic Effects through a Tagged Reporter Protein
Author(s) -
CamachoZarco Aldo R.,
Munari Francesca,
Wegstroth Melanie,
Liu WeiMin,
Ubbink Marcellus,
Becker Stefan,
Zweckstetter Markus
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201408615
Subject(s) - paramagnetism , chemistry , computational biology , biology , physics , condensed matter physics
Abstract Paramagnetic effects provide unique information about the structure and dynamics of biomolecules. We developed a method in which the lanthanoid tag is not directly attached to the protein of interest, but instead to a “reporter” protein, which binds and then transmits paramagnetic information to the target. The designed method allows access to a large number of paramagnetic restraints and residual dipolar couplings produced from independent molecular alignments in high‐molecular‐weight proteins with unknown 3D structure