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Multiplex Detection of Enzymatic Activity with Responsive Lanthanide‐Based Luminescent Probes
Author(s) -
Pershagen Elias,
Borbas K. Eszter
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201408560
Subject(s) - analyte , multiplex , lanthanide , modularity (biology) , luminescence , nanotechnology , chemistry , materials science , chromatography , bioinformatics , optoelectronics , biology , organic chemistry , ion , genetics
Abstract Multiplex analyte detection in complex dynamic systems is desirable for the investigation of cellular communication networks as well as in medical diagnostics. A family of lanthanide‐based responsive luminescent probes for multiplex detection is reported. The high modularity of the probe design enabled the rapid assembly of both green and red emitters for a large variety of analytes by the simple exchange of the lanthanide or an analyte‐cleavable caging group, respectively. The real‐time three‐color detection of up to three analytes was demonstrated, thus setting the stage for the non‐invasive investigation of interconnected biological processes.