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Growth and Origami Folding of DNA on Nanoparticles for High‐Efficiency Molecular Transport in Cellular Imaging and Drug Delivery
Author(s) -
Yan Juan,
Hu Chongya,
Wang Ping,
Zhao Bin,
Ouyang Xiangyuan,
Zhou Juan,
Liu Rui,
He Dang,
Fan Chunhai,
Song Shiping
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201408247
Subject(s) - superstructure , nanoparticle , folding (dsp implementation) , nanotechnology , intercalation (chemistry) , molecule , dna , materials science , mechanism (biology) , drug delivery , biophysics , colloidal gold , rolling circle replication , dna origami , small molecule , chemistry , nanostructure , biochemistry , inorganic chemistry , oceanography , philosophy , dna replication , engineering , organic chemistry , epistemology , geology , electrical engineering , biology
A novel three‐dimensional (3D) superstructure based on the growth and origami folding of DNA on gold nanoparticles (AuNPs) was developed. The 3D superstructure contains a nanoparticle core and dozens of two‐dimensional DNA belts folded from long single‐stranded DNAs grown in situ on the nanoparticle by rolling circle amplification (RCA). We designed two mechanisms to achieve the loading of molecules onto the 3D superstructures. In one mechanism, ligands bound to target molecules are merged into the growing DNA during the RCA process (merging mechanism). In the other mechanism, target molecules are intercalated into the double‐stranded DNAs produced by origami folding (intercalating mechanism). We demonstrated that the as‐fabricated 3D superstructures have a high molecule‐loading capacity and that they enable the high‐efficiency transport of signal reporters and drugs for cellular imaging and drug delivery, respectively.