Premium
Recruitment of SH‐Containing Peptides to Lipid and Biological Membranes through the Use of a Palmitic Acid Functionalized with a Maleimide Group
Author(s) -
Haralampiev Ivan,
Mertens Monique,
Schwarzer Roland,
Herrmann Andreas,
Volkmer Rudolf,
Wessig Pablo,
Müller Peter
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201408089
Subject(s) - maleimide , membrane , palmitic acid , biological membrane , chemistry , biomolecule , rhodamine , liposome , peptide , rhodamine b , fluorescence spectroscopy , fluorescence , biophysics , biochemistry , organic chemistry , fatty acid , biology , physics , quantum mechanics , photocatalysis , catalysis
This study presents a novel and easily applicable approach to recruit sulfhydryl‐containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine‐labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.