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Ultrafast Extraction of Proteins from Tissues Using Desorption by Impulsive Vibrational Excitation
Author(s) -
Kwiatkowski Marcel,
Wurlitzer Marcus,
Omidi Maryam,
Ren Ling,
Kruber Sebastian,
Nimer Refat,
Robertson Wesley D.,
Horst Andrea,
Miller R. J. Dwayne,
Schlüter Hartmut
Publication year - 2015
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201407669
Subject(s) - picosecond , desorption , excitation , chemistry , intramolecular force , millisecond , ultrashort pulse , molecule , extraction (chemistry) , laser , analytical chemistry (journal) , chromatography , stereochemistry , adsorption , organic chemistry , optics , physics , astronomy , electrical engineering , engineering
A picosecond IR laser (PIRL) can be used to blast proteins out of tissues through desorption by impulsive excitation (DIVE) of intramolecular vibrational states of water molecules in the cell in less than a millisecond. With PIRL‐DIVE proteins covering a range of a few kDa up to several MDa are extracted in high quantities compared to conventional approaches. The chemical composition of extracted proteins remains unaltered and even enzymatic activities are maintained.