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Selective Transcription of an Unnatural Naphthyridine:Imidazopyridopyrimidine Base Pair Containing Four Hydrogen Bonds with T7 RNA Polymerase
Author(s) -
Nomura Yusaku,
Kashiwagi Satoshi,
Sato Kousuke,
Matsuda Akira
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201406402
Subject(s) - base pair , transcription (linguistics) , t7 rna polymerase , chemistry , polymerase , ribonucleoside , dna , rna polymerase ii , hydrogen bond , microbiology and biotechnology , rna , biology , gene , biochemistry , promoter , molecule , gene expression , linguistics , philosophy , organic chemistry , escherichia coli , bacteriophage
The naphthyridine:imidazopyridopyrimidine base pair is the first base pair containing four hydrogen bonds that can be replicated selectively and efficiently by the use of DNA polymerases. Herein we describe the synthesis of naphthyridine‐ C ‐ribonucleoside 5′‐triphosphate (rNaTP) and transcription reactions catalyzed by T7 RNA polymerase with rNaTP and template DNA containing imidazopyridopyrimidine. The transcription reaction was also applied to a longer transcript containing part of the human c‐Ha‐Ras gene.