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Bioorthogonal Cleavage and Exchange of Major Histocompatibility Complex Ligands by Employing Azobenzene‐Containing Peptides
Author(s) -
Choo Joanna A. L.,
Thong Sock Yue,
Yap Jiawei,
van Esch Wim J. E.,
Raida Manfred,
Meijers Rob,
Lescar Julien,
Verhelst Steven H. L.,
Grotenbreg Gijsbert M.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201406295
Subject(s) - bioorthogonal chemistry , azobenzene , chemistry , sodium dithionite , major histocompatibility complex , epitope , cleavage (geology) , combinatorial chemistry , biochemistry , antigen , biology , molecule , click chemistry , genetics , organic chemistry , paleontology , fracture (geology) , gene
Bioorthogonal cleavable linkers are attractive building blocks for compounds that can be manipulated to study biological and cellular processes. Sodium dithionite sensitive azobenzene‐containing (Abc) peptides were applied for the temporary stabilization of recombinant MHC complexes, which can then be employed to generate libraries of MHC tetramers after exchange with a novel epitope. This technology represents an important tool for high‐throughput studies of disease‐specific T cell responses.