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Imaging Lipid Metabolism in Live Caenorhabditis elegans Using Fingerprint Vibrations
Author(s) -
Wang Ping,
Liu Bin,
Zhang Delong,
Belew Micah Y.,
Tissenbaum Heidi A.,
Cheng JiXin
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201406029
Subject(s) - caenorhabditis elegans , degree of unsaturation , organelle , chemical imaging , chemistry , lipid metabolism , hyperspectral imaging , biophysics , biochemistry , biology , chromatography , gene , remote sensing , geology
Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long‐standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome‐related organelles in intestinal cells are sites for storage of cholesterol in C. elegans .