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Dissolution‐Enhanced Luminescent Bioassay Based on Inorganic Lanthanide Nanoparticles
Author(s) -
Zhou Shanyong,
Zheng Wei,
Chen Zhuo,
Tu Datao,
Liu Yongsheng,
Ma En,
Li Renfu,
Zhu Haomiao,
Huang Mingdong,
Chen Xueyuan
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201405937
Subject(s) - lanthanide , luminescence , detection limit , dissolution , biomolecule , nanoparticle , bioassay , immunoassay , chemistry , luminescent measurements , biocompatibility , fluorescence , materials science , nanotechnology , nuclear chemistry , chromatography , ion , biology , organic chemistry , immunology , physics , optoelectronics , quantum mechanics , antibody , genetics
Conventional dissociation‐enhanced lanthanide fluoroimmunoassays (DELFIA) using molecular probes suffer from a low labeling ratio of lanthanide ions (Ln 3+ ) per biomolecule. Herein, we develop a unique bioassay based on the dissolution‐enhanced luminescence of inorganic lanthanide nanoparticles (NPs). As a result of the highly concentrated Ln 3+ ions in a single Ln 3+ NP, an extremely high Ln 3+ labeling ratio can be achieved, which amplifies significantly the luminescence signal and thus improves the detection sensitivity compared to DELFIA. Utilizing sub‐10 nm NaEuF 4 NPs as dissolution‐enhanced luminescent nanoprobes, we demonstrate the successful in vitro detection of carcinoembryonic antigen (CEA, an important tumor marker) in human serum samples with a record‐low detection limit of 0.1 pg mL −1 (0.5 f M ). This value is an improvement of approximately 3 orders of magnitude relative to that of DELFIA. The dissolution‐enhanced luminescent bioassay shows great promise in versatile bioapplications, such as ultrasensitive and multiplexed in vitro detection of disease markers in clinical diagnosis.