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Sequential Gene Silencing Using Wavelength‐Selective Caged Morpholino Oligonucleotides
Author(s) -
Yamazoe Sayumi,
Liu Qingyang,
McQuade Lindsey E.,
Deiters Alexander,
Chen James K.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201405355
Subject(s) - morpholino , oligonucleotide , zebrafish , gene silencing , embryo , biology , microbiology and biotechnology , gene , chemistry , computational biology , genetics
Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength‐selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse‐genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.