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Development of Multinuclear Polymeric Nanoparticles as Robust Protein Nanocarriers
Author(s) -
Wu Jun,
Kamaly Nazila,
Shi Jinjun,
Zhao Lili,
Xiao Zeyu,
Hollett Geoffrey,
John Rohit,
Ray Shaunak,
Xu Xiaoyang,
Zhang Xueqing,
Kantoff Philip W.,
Farokhzad Omid C.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201404766
Subject(s) - nanocarriers , nanoparticle , plga , nanotechnology , aqueous solution , chemistry , nanocapsules , chemical engineering , surface charge , biophysics , drug delivery , homogeneous , materials science , organic chemistry , physics , engineering , biology , thermodynamics
One limitation of current biodegradable polymeric nanoparticles is their inability to effectively encapsulate and sustainably release proteins while maintaining protein bioactivity. Here we report the engineering of PLGA–polycation nanoparticles with a core–shell structure that act as a robust vector for the encapsulation and delivery of proteins and peptides. The optimized nanoparticles can load high amounts of proteins (>20 % of nanoparticles by weight) in aqueous solution without organic solvents through electrostatic interactions by simple mixing, thereby forming nanospheres in seconds with diameters <200 nm. The relationship between nanosphere size, surface charge, PLGA–polycation composition, and protein loading is also investigated. The stable nanosphere complexes contain multiple PLGA–polycation nanoparticles, surrounded by large amounts of protein. This study highlights a novel strategy for the delivery of proteins and other relevant molecules.