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Cell‐Surface Engineering by a Conjugation‐and‐Release Approach Based on the Formation and Cleavage of Oxime Linkages upon Mild Electrochemical Oxidation and Reduction
Author(s) -
Pulsipher Abigail,
Dutta Debjit,
Luo Wei,
Yousaf Muhammad N.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201404099
Subject(s) - oxime , cleavage (geology) , electrochemistry , chemistry , reduction (mathematics) , combinatorial chemistry , cell , redox , chemical engineering , biophysics , electrode , organic chemistry , materials science , biochemistry , composite material , engineering , biology , fracture (geology) , geometry , mathematics
We report a strategy to rewire cell surfaces for the dynamic control of ligand composition on cell membranes and the modulation of cell–cell interactions to generate three‐dimensional (3D) tissue structures applied to stem‐cell differentiation, cell‐surface tailoring, and tissue engineering. We tailored cell surfaces with bioorthogonal chemical groups on the basis of a liposome‐fusion and ‐delivery method to create dynamic, electroactive, and switchable cell‐tissue assemblies through chemistry involving chemoselective conjugation and release. Each step to modify the cell surface: activation, conjugation, release, and regeneration, can be monitored and modulated by noninvasive, label‐free analytical techniques. We demonstrate the utility of this methodology by the conjugation and release of small molecules to and from cell surfaces and by the generation of 3D coculture spheroids and multilayered cell tissues that can be programmed to undergo assembly and disassembly on demand.