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Optical‐Resolution Photoacoustic Microscopy for Volumetric and Spectral Analysis of Histological and Immunochemical Samples
Author(s) -
Zhang Yu Shrike,
Yao Junjie,
Zhang Chi,
Li Lei,
Wang Lihong V.,
Xia Younan
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201403812
Subject(s) - microscopy , materials science , resolution (logic) , biomedical engineering , optics , chromogenic , photoacoustic spectroscopy , photoacoustic imaging in biomedicine , sample preparation , chemistry , computer science , chromatography , artificial intelligence , medicine , physics
Optical‐resolution photoacoustic microscopy (OR‐PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR‐PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR‐PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR‐PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR‐PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.