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Pattern‐Based Detection of Toxic Metals in Surface Water with DNA Polyfluorophores
Author(s) -
Yuen Lik Hang,
Franzini Raphael M.,
Wang Shenliang,
Crisalli Pete,
Singh Vijay,
Jiang Wei,
Kool Eric T.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201403235
Subject(s) - contamination , tetramer , metal , metal ions in aqueous solution , analyte , fluorescence , environmental chemistry , contaminated water , surface water , chemistry , heavy metals , water contamination , nanotechnology , materials science , environmental science , chromatography , biology , organic chemistry , environmental engineering , ecology , physics , quantum mechanics , enzyme
Heavy metal contamination of water can be toxic to humans and wildlife; thus the development of methods to detect this contamination is of high importance. Here we describe the design and application of DNA‐based fluorescent chemosensors on microbeads to differentiate eight toxic metal ions in water. We developed and synthesized four fluorescent 2′‐deoxyribosides of metal‐binding ligands. A tetramer‐length oligodeoxy‐fluoroside (ODF) library of 6561 members was constructed and screened for sequences responsive to metal ions, of which seven sequences were selected. Statistical analysis of the response patterns showed successful differentiation of the analytes at concentrations as low as 100 n M . Sensors were able to classify water samples from 13 varied sites and quantify metal contamination in unknown specimens. The results demonstrate the practical potential of bead‐based ODF chemosensors to analyze heavy metal contamination in water samples by a simple and inexpensive optical method.