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Engineering Specificity Changes on a RanBP2 Zinc Finger that Binds Single‐Stranded RNA
Author(s) -
Vandevenne Marylène,
O'Connell Mitchell R.,
Helder Stephanie,
Shepherd Nicholas E.,
Matthews Jacqueline M.,
Kwan Ann H.,
Segal David J.,
Mackay Joel P.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201402980
Subject(s) - zinc finger , rna , computational biology , rna binding protein , biology , transcription (linguistics) , genetics , gene , chemistry , microbiology and biotechnology , transcription factor , linguistics , philosophy
The realization that gene transcription is much more pervasive than previously thought and that many diverse RNA species exist in simple as well as complex organisms has triggered efforts to develop functionalized RNA‐binding proteins (RBPs) that have the ability to probe and manipulate RNA function. Previously, we showed that the RanBP2‐type zinc finger (ZF) domain is a good candidate for an addressable single‐stranded‐RNA (ssRNA) binding domain that can recognize ssRNA in a modular and specific manner. In the present study, we successfully engineered a sequence specificity change onto this ZF scaffold by using a combinatorial approach based on phage display. This work constitutes a foundation from which a set of RanBP2 ZFs might be developed that is able to recognize any given RNA sequence.